Blood Typing

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The whole blood typing process is available as a RealPlayer video.

The Whole Process 1.86 MB 12 min

Or, to see a specific segment, click on a picture to view the corresponding movie.

QuickTime RealPlayer
View QuickTime movie No Food or Drinks 0:30 View RealPlayer file
Because of the danger of contamination with blood, all soft drinks, candy, and food should be removed from the lab or put safely away in your bookbag before the lab begins.
View QuickTime movie Use Gloves When Helping 0:19 View RealPlayer file
To reduce the danger of contamination, you should do your own blood typing. If it becomes necessary to help someone else, you must wear gloves for your own protection.
View QuickTime movie Antisera 0:24 View RealPlayer file
The antisera should be at room temperature when they are used. The blue antiserum is anti-A antibodies, the yellow one is anti-B, and the “clear” one is anti-D (anti-Rh+).
View QuickTime movie Equipment Used 1:16 View RealPlayer file
Insure that all equipment listed in your lab protocol is available and ready for use.
View QuickTime movie Marking the Slide 0:41 View RealPlayer file
A wax pencil should be used to “divide” a slide into thirds and draw a large circle in each third. The circles should be labeled (“A,” “B,” or “D,”) and the slide should be initialed.
View QuickTime movie Loading the Lancet 0:37 View RealPlayer file
The lancet should be inserted into the Autolet® and the tip guard carefully removed. After the Autolet® is “closed,” an orange finger platform should be inserted.
View QuickTime movie Adding Antisera to Slide 1:15 View RealPlayer file
A drop of the appropriate antiserum should be placed just inside one edge of the appropriate circle. Care should be taken that the antisera are not cross-contaminated.
View QuickTime movie Sterilizing Finger 0:24 View RealPlayer file
The appropriate finger should be sterilized with a Kimwipe® and 70% ethanol and allowed to air-dry. If you are right-handed and do not play guitar, etc., the ring finger of your left hand should be used.
View QuickTime movie Pricking Finger 2:10 View RealPlayer file
Hold hand below your heart. Working quickly, lance the fleshy side of the tip on the little-finger side, place a drop of blood inside the opposite edge of each circle, then stir each with its own toothpick.
View QuickTime movie Rh Viewbox 0:40 View RealPlayer file
Place your slide on the Rh viewbox to warm it, and gently rock the viewbox back and forth to help the antisera to react with any antigens present on your RBCs.
View QuickTime movie Proper Disposal of Trash 1:26 View RealPlayer file
While your slide is reacting, THREE toothpicks, the lancet cover, and any paper should be properly disposed of. The orange finger platform and the lancet should be ejected into the correct containers.
View QuickTime movie Nothing in Trash Can 0:27 View RealPlayer file
Lancets should never be placed into the regular trash because someone could get stuck. Lancets should only be placed in the specially-marked sharps container.
View QuickTime movie Agglutination 0:17 View RealPlayer file
Examine your slide for agglutination. A graininess in any of the circles is a reaction that indicated that you have that blood type. Record these data in your lab notebook.
View QuickTime movie Slide into Bleach Solution 0:31 View RealPlayer file
Gently place your used slide into a 10% solution of chlorine bleach to disinfect it (lab personnel will complete the process). Wearing goggles can prevent eye injury due to splashed bleach solution.
View QuickTime movie End-of-Day Clean-Up 1:18 View RealPlayer file
Before you leave, make sure all blood-stained items are correctly disposed of. Other classes should be warned to consume no food in the lab room until the tabletops have been treated with bleach-water.

Further Information Relating to Blood Typing

Genetics Background
Antigens and Antibodies

Submit Your Blood Type Data

A | B | AB | O
Rh+ | Rh-

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View Class Data

Copyright © 1999 by J. Stein Carter and A. Gregory. All rights reserved.
This page has been accessed Counter times since 9 Jan 2003.